Comparison of Responses of the Clone 4430 and Tradescantia pallida in the Micronucleus Assay (Trad-MN)

Authors

  • Ana Cristina Mielli Laboratório de Poluição Atmosférica Experimental, Faculdade de Medicina, Universidade de São Paulo (USP), São Paulo, SP, Brazil
  • Paulo Hilário Nascimento Saldiva Laboratório de Ecotoxicologia Aquática e Limnologia, Faculdade de Tecnologia, Universidade Estadual de Campinas (UNICAMP), Campinas, SP, Brazil
  • Gisela de Aragão Umbuzeiro Laboratório de Ecotoxicologia Aquática e Limnologia, Faculdade de Tecnologia, Universidade Estadual de Campinas (UNICAMP), Campinas, SP, Brazil

DOI:

https://doi.org/10.5132/jbse.2009.01.007

Keywords:

Clone 4430, environmental genotoxicity, Micronuclei (MN), Tradescantia pallida

Abstract

The Genotoxicity Assay with clone 4430 genus Tradescantia is utilized worldwide in the Tradescantia Micronucleus assay (Trad-MN). In Brazil, several Trad-MN studies have utilized Tradescantia pallida to replace the clone 4430. However, in the literature, only one comparison between the two species was performed, and more studies are necessary to examine the mutagenic potential of Tradescantia pallida for application in the Trad-MN. The present work aims to compare the results of Trad-MN assays between the two plants, in the testing of mutagenic substances (positive controls) and water (as negative controls), to evaluate the utilization potential of Tradescantia pallida for replacing clone 4430. The results obtained showed that Tradescantia pallida can replace clone 4430 in the micronucleus assay, however, the substances testing showed high variation in the micronuclei induction (3%–8% for negative control and 5%–11% for positive control), indicating low discriminating potential of the assay and limited applicability in monitoring programs.

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Published

20-06-2009

How to Cite

Mielli, A. C., Saldiva, P. H. N., & Umbuzeiro, G. de A. (2009). Comparison of Responses of the Clone 4430 and Tradescantia pallida in the Micronucleus Assay (Trad-MN). Ecotoxicology and Environmental Contamination, 4(1), 49–54. https://doi.org/10.5132/jbse.2009.01.007

Issue

Vol. 4 No. 1 (2009)

Section

Original Articles

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Copyright (c) 2009 Ecotoxicology and Environmental Contamination

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