Evaluation of Genotoxicity Induced by Atmospheric Pollution Using the Land Mollusk Cantareus aspersus (Müller, 1774) as Biomonitor and the Comet Assay as Biomarker

Authors

  • Juliana da Silva Laboratório de Genética Toxicológica, PPGGTA, Universidade Luterana do Brasil, Canoas, RS, Brazil
  • N. Simeoni Laboratório de Genética Toxicológica, PPGGTA, Universidade Luterana do Brasil, Canoas, RS, Brazil
  • A. A. Groff Laboratório de Genética Toxicológica, PPGGTA, Universidade Luterana do Brasil, Canoas, RS, Brazil
  • M. Ianistcki Laboratório de Genética Toxicológica, PPGGTA, Universidade Luterana do Brasil, Canoas, RS, Brazil
  • V. Benvegnú Laboratório de Genética Toxicológica, PPGGTA, Universidade Luterana do Brasil, Canoas, RS, Brazil
  • N. T. SCHRÖDER Laboratório de Poluição Ambiental, PPGEAM, Universidade Luterana do Brasil, Canoas, RS, Brazil

DOI:

https://doi.org/10.5132/jbse.2007.01.007

Keywords:

biomonitoring, Comet Assay, Cantareus aspersus, atmospheric pollution

Abstract

The objective of this work was to investigate the viability of using the land mollusk Cantareus aspersus (Müller, 1774) as biomonitor and the Comet Assay as biomarker, exposing organisms to atmospheric pollutants. Organisms were exposed to air at the Ulbra University campus climatic control station (Canoas, RS) for 7, 15 and 30 days, in the winter/2004 and in the summer/2005. Our control group, not exposed, was kept in the laboratory, under controlled conditions. Lung cells and hemolymph cells were evaluated through Comet Assay, which shows sensitive results in a short time in quantifying lesions and detecting DNA repair effects. 100 cells/individual/tissue were analyzed and visually classified into five classes (0 – no effect to 4 – maximum damage). Results show that pulmonary cells are more sensitive than hemolymph cells. Longer exposition time produced more DNA damage. Considering climatic seasons, no significant differences were detected between them. Both organisms and method showed to be cheap, effective and sensitive in our study, for biomonitoring atmospheric pollution.

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Published

10-03-2007

How to Cite

Silva, J. da, Simeoni, N., Groff, A. A., Ianistcki, M., Benvegnú, V., & SCHRÖDER, N. T. (2007). Evaluation of Genotoxicity Induced by Atmospheric Pollution Using the Land Mollusk Cantareus aspersus (Müller, 1774) as Biomonitor and the Comet Assay as Biomarker. Ecotoxicology and Environmental Contamination, 2(1), 45–51. https://doi.org/10.5132/jbse.2007.01.007

Issue

Vol. 2 No. 1 (2007)

Section

Original Articles

License

Copyright (c) 2007 Ecotoxicology and Environmental Contamination

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