Evaluation of Genotoxicity Induced by Atmospheric Pollution Using the Land Mollusk Cantareus aspersus (Müller, 1774) as Biomonitor and the Comet Assay as Biomarker

Autores/as

  • Juliana da Silva Laboratório de Genética Toxicológica, PPGGTA, Universidade Luterana do Brasil, Canoas, RS, Brazil
  • N. Simeoni Laboratório de Genética Toxicológica, PPGGTA, Universidade Luterana do Brasil, Canoas, RS, Brazil
  • A. A. Groff Laboratório de Genética Toxicológica, PPGGTA, Universidade Luterana do Brasil, Canoas, RS, Brazil
  • M. Ianistcki Laboratório de Genética Toxicológica, PPGGTA, Universidade Luterana do Brasil, Canoas, RS, Brazil
  • V. Benvegnú Laboratório de Genética Toxicológica, PPGGTA, Universidade Luterana do Brasil, Canoas, RS, Brazil
  • N. T. SCHRÖDER Laboratório de Poluição Ambiental, PPGEAM, Universidade Luterana do Brasil, Canoas, RS, Brazil

DOI:

https://doi.org/10.5132/jbse.2007.01.007

Palabras clave:

biomonitoring, Comet Assay, Cantareus aspersus, atmospheric pollution

Resumen

The objective of this work was to investigate the viability of using the land mollusk Cantareus aspersus (Müller, 1774) as biomonitor and the Comet Assay as biomarker, exposing organisms to atmospheric pollutants. Organisms were exposed to air at the Ulbra University campus climatic control station (Canoas, RS) for 7, 15 and 30 days, in the winter/2004 and in the summer/2005. Our control group, not exposed, was kept in the laboratory, under controlled conditions. Lung cells and hemolymph cells were evaluated through Comet Assay, which shows sensitive results in a short time in quantifying lesions and detecting DNA repair effects. 100 cells/individual/tissue were analyzed and visually classified into five classes (0 – no effect to 4 – maximum damage). Results show that pulmonary cells are more sensitive than hemolymph cells. Longer exposition time produced more DNA damage. Considering climatic seasons, no significant differences were detected between them. Both organisms and method showed to be cheap, effective and sensitive in our study, for biomonitoring atmospheric pollution.

Descargas

Publicado

2007-03-10

Cómo citar

Silva, J. da, Simeoni, N., Groff, A. A., Ianistcki, M., Benvegnú, V., & SCHRÖDER, N. T. (2007). Evaluation of Genotoxicity Induced by Atmospheric Pollution Using the Land Mollusk Cantareus aspersus (Müller, 1774) as Biomonitor and the Comet Assay as Biomarker. Ecotoxicology and Environmental Contamination, 2(1), 45–51. https://doi.org/10.5132/jbse.2007.01.007

Número

Vol. 2 Núm. 1 (2007)

Sección

Original Articles

Licencia

Derechos de autor 2007 Ecotoxicology and Environmental Contamination

Creative Commons License

Esta obra está bajo una licencia internacional Creative Commons Atribución 4.0.

Copyright © 2006 ECOTOX-Brasil

Copyright notice: It is a condition for publication that manuscripts submitted to this journal have not yet been published and will not be simultaneously submitted or published elsewhere. By submitting a manuscript, the authors agree that copyright for their article is transferred to the Sociedade Brasileira de Ecotoxicologia (ECOTOX-Brasil) if and when the article is accepted for publication. The copyright covers the exclusive rights to reproduce and distribute articles, including reprints, photographic reproductions or any other reproduction of a similar nature, including translations. No part of this publication may be reproduced, stored in a retrieval system or transmitted in any form or by any means, electronic, mechanical, photocopying, recording or otherwise, without permission of the publisher.

Notice: While every effort is made by the EEC, editors and editorial board to see that no inaccurate or misleading data, opinions or statements appear in this journal, they wish to make it clear that the contents of the articles and advertisements published herein are the sole responsibility of the contributors or advertisers concerned. Accordingly, the EEC, the editorial board and editors and their respective employees, officers and agents accept no responsibility or liability whatsoever for the consequences of any inaccurate or misleading data, opinion or statement.